DNA
R6K

Part:BBa_J61001:Design

Designed by: John Anderson   Group: Arkin Lab   (2006-07-31)


[R6K] Origin of replication


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

PCR ca1011F/R on pG80ko (445 bp, EcoRI/SpeI)

Sub into pSB1A2-I13522 (EcoRI/SpeI)

Product is Bca1011

ca1011F Forward Biobricking of R6K

GGACTgaattcgcggccgcttctagagtgattcgcacgggcccatg

ca1011R Reverse biobricking of R6K

ccgctactagtaGCAGTTCAACCTGTTGATAG

References